Larix sibirica Genome sequencing and assembly

The server version of CLC Assembly Cell was used to assemble de novo Siberian larch (Larix sibirica) genome using the Illumina PE and MP reads. A stepwise approach was developed and used to assemble this large genome (12.03 Gb). It is described in Kuzmin et al. BMC Bioinformatics 2019 20(Suppl 1): 37 ( https://doi.org/10.1186/s12859-018-2570-y). The assembly took 23 days of computing. The number of obtained scaffolds is 11.3 million with a total length of 12.34 Gb. The longest scaffold is 354 Kb, N50 is 6443.本研究借助CLC Assembly Cell服务器版软件，利用Illumina PE与MP测序读段，对西伯利亚落叶松（Larix sibirica）进行从头（de novo）基因组组装。针对该12.03 Gb的大型基因组，我们开发并应用了分步组装策略，该策略的详细信息已发表于Kuzmin等人的研究：《BMC Bioinformatics》2019年，20（增刊1）：37（DOI: 10.1186/s12859-018-2570-y）。本次组装的计算时长总计23天，最终共获得1130万条支架序列（scaffold），总长度为12.34 Gb；最长支架序列长度达354 Kb，组装连续性指标N50为6443。