SmcHD1,H3k27me3,CTCF and RAd21 ChIP-seq

In this study we investigate the role of the non-canonical SMC family protein, SmcHD1in the X inactivation. Set of allele-specific ChIP-seq experiments on female clonal inter-specific (M.m.domesticus FVB x M.m.Castaneus) MEF cell lines: wild-type MEFs, SmcHD1 MomeD1 mut MEFs (SmcHD1 null) and SmcHD1 CRISPR KO MEFs (derived from wild-type MEFs after establishemnt of X inactivation). Each ChIP library was accompanied with respective input library and each experiment was done in biological replicates (2-4).本研究探讨了非经典结构维持染色体（Structural Maintenance of Chromosomes, SMC）家族蛋白SmcHD1在X染色体失活（X inactivation）中的作用。本研究针对雌性克隆种间（小家鼠Domesticus亚种FVB品系 × 小家鼠Castaneus亚种）小鼠胚胎成纤维细胞（Mouse Embryo Fibroblasts, MEF）细胞系开展了系列等位基因特异性染色质免疫共沉淀测序（ChIP-seq）实验，所用细胞系涵盖野生型MEF、SmcHD1 MomeD1突变型MEF（SmcHD1基因敲除型，SmcHD1 null）以及SmcHD1 CRISPR敲除型MEF（该细胞系由X染色体失活建立完成后的野生型MEF衍生而来）。每一组ChIP文库均配有对应的输入文库（input library），且每项实验均设置了2至4次生物学重复。