Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts [triplicates]. Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts [triplicates]

To investigate the cooperative function of dermal fibroblast and mast cell in inducing mast cell tolerance to commensals We then performed gene expression profiling analysis using data obtained from RNA-seq of mast cells, and mast cells conditioned by dermal fibroblasts before and after treatments with commensal bacteria supernatants. Overall design: Comparative gene expression profiling analysis of RNA-seq data for conditioned and not conditioned mast cells

为探究真皮成纤维细胞（dermal fibroblast）与肥大细胞（mast cell）在诱导肥大细胞对共生菌（commensals）产生耐受过程中的协同功能，我们采用两类RNA测序（RNA-seq）数据开展基因表达谱分析：一类来自未处理的肥大细胞，另一类来自经真皮成纤维细胞预处理后，再经共生菌上清液处理前后的肥大细胞。
整体实验设计：对经预处理与未预处理的肥大细胞的RNA测序数据进行基因表达谱对比分析。