HDAC1 Frameshiftting IP mass spectrometry

In eukaryotes, several gene have been found that ribosomal frameshifting efficiently occurs on its slippery site. However, less study focuses on codon repeat induce frameshifting. To screen whether the codon repeat in HDAC1 gene could induced frameshifting and produce frameshifting peptide. we generate a HDAC1-FS-Flag construct. Detection the HDAC1 frameshifting protein using immunoprecipitation-Mass Spectrometry.在真核生物（eukaryotes）中，已有研究证实多个基因的滑动位点（slippery site）可高效发生核糖体移码（ribosomal frameshifting）。然而，针对密码子重复（codon repeat）诱导移码的相关研究仍相对较少。为探究HDAC1基因中的密码子重复是否可诱导移码并产生移码肽（frameshifting peptide），我们构建了HDAC1-FS-Flag构建体，并采用免疫沉淀-质谱联用（immunoprecipitation-Mass Spectrometry）技术对HDAC1移码蛋白进行检测。