Gene expression profile of murine Flvcr-deleted and control CD4+CD8+ double-positive (DP) thymocytes. Mus musculus

Genomewide microaarray analysis of murine DP thymocytes to determine the genes whose expression was altered by FLVCR loss Gene signatures from Flvcr+/+ DP thymocytes was compared to gene signatures from Flvcr-/- DP thymocytes Overall design: Total RNA obtained from sort-purified Flvcr-/- and Flvcr+/+ DP thymocytes

为明确受FLVCR缺失影响而发生表达改变的基因，本研究对小鼠双阳性胸腺细胞（double positive thymocytes, DP）开展全基因组微阵列分析。将野生型（Flvcr+/+）双阳性胸腺细胞的基因表达特征与纯合缺失型（Flvcr-/-）双阳性胸腺细胞的基因表达特征进行对比。实验设计：样本取自经分选纯化的Flvcr-/-与Flvcr+/+双阳性胸腺细胞，提取总RNA。