Transcriptomic analysis reveals distinct adaptive molecular mechanism in the hippocampal CA3 from rats susceptible or not-susceptible to hyperthermia-induced seizures. Transcriptomic analysis reveals distinct adaptive molecular mechanism in the hippocampal CA3 from rats susceptible or not-susceptible to hyperthermia-induced seizures

This study aimed to identify the genomic differences - through differential gene expression, miRNAs, and WGCNA transcriptional modules among animals with or without seizure after insult and non-insulted controls. Overall design: Use of a rat (Rattus norvegicus) model of hyperthermia-induced seizure to characterize the genomic mechanism underlying febrile seizures in epilepsy. The aim of the study is to identify the genomic differences in hippocampal ventral CA3 region - through differential gene expression, miRNAs, and WGCNA transcriptional modules among animals with or without seizure after insult and non-insulted controls (which would be impossible to study in humans). Here, samples from the animal with seizure.

本研究旨在通过差异基因表达（differential gene expression）、微小RNA（miRNAs）以及加权基因共表达网络分析（WGCNA）转录模块，对比致伤后伴或不伴癫痫发作的动物与非致伤对照组之间的基因组差异。
整体实验设计：采用热诱导癫痫发作的大鼠（Rattus norvegicus）模型，以解析癫痫中热性惊厥的潜在基因组机制。
本研究的核心目标为，在海马腹侧CA3区中，通过差异基因表达、miRNAs以及WGCNA转录模块，对比致伤后伴或不伴癫痫发作的动物与非致伤对照组之间的基因组差异——此类研究在人类中无法开展。
本次实验的样本取自致伤后出现癫痫发作的动物。