Clonally expanded targetable natural killer-like T cells seed the aged spinal cord to disrupt wound healing. Clonally expanded targetable natural killer-like T cells seed the aged spinal cord to disrupt wound healing

CD8+ T cells that were found accumulated in the spinal cord preceding (aged) and following a spinal cord injury disrupt wound healing. We isolated spinal cord cells of the aged mice who were treated with IgG or anti-CD8 monoclonal antibody following spinal cord injury and transcriptionally profiled them by doing single cell RNAseq to identify what cells were affected after depleting CD8+ T cells. Overall design: Aged mouse spinal cord segments were collected from IgG or anti-CD8 antibody conditions 28 days after spinal cord injury. After, cells were isolated for single cell RNAseq. Two biological replicates were included in each condition.

研究表明，在老年小鼠脊髓损伤前后聚集于脊髓的CD8+ T细胞会阻碍伤口愈合。本研究对脊髓损伤后接受IgG或抗CD8单克隆抗体（anti-CD8 monoclonal antibody）治疗的老年小鼠的脊髓细胞进行分离，并通过单细胞RNA测序（single cell RNAseq）开展转录组分析，以明确CD8+ T细胞耗竭后哪些细胞的基因表达发生改变。实验整体设计：脊髓损伤后28天，分别从接受IgG治疗及抗CD8抗体治疗的老年小鼠中采集脊髓节段，随后分离细胞用于单细胞RNA测序。每个处理组均包含2个生物学重复。