High-throughput yeast CRISPR genome editing. Multiplexed Precision Genome Editing with Trackable, Genomic Barcodes

Here we describe a CRISPR/Cas9-based platform in S. cerevisiae for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC), which employs array-synthesized guide-donor oligos for plasmid-based high-throughput editing and features genomic barcode integration to prevent plasmid barcode loss and enable robust phenotyping.

本文介绍了一种应用于酿酒酵母（Saccharomyces cerevisiae）的基于CRISPR/Cas9的多重精准基因组编辑与可追踪整合细胞条形码平台（MAGESTIC）。该平台采用阵列合成的向导-供体寡核苷酸（guide-donor oligos）开展基于质粒的高通量基因编辑，具备基因组条形码整合特性，可防止质粒条形码丢失并实现稳定可靠的表型分析。