Expression analysis of Spathaspora passalidarum NRRL Y-27907 grown in glucose or xylose

Investigation of whole genome gene expression level changes in Spathaspora passalidarum NRRL Y-27907 grown aerobically in xylose, compared to the same strain grown aerobically in glucose. A six array study using total RNA recovered from three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in glucose and three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in xylose. Each array measures the expression level of 362,487 probes (average probe length 54.5 +/- 4.0 nt) tiled across the Spathaspora passalidarum NRRL Y-27907 genome with a median spacing distance of 29 nt. During data processing, probes are filtered to include only those probes corresponding to annotated protein-coding genes.

本研究针对帕拉斯孔斯孢酵母（Spathaspora passalidarum）NRRL Y-27907展开分析，对比该菌株在好氧木糖培养与好氧葡萄糖培养两种条件下的全基因组基因表达水平变化。本实验采用6张基因芯片开展研究，分别从3份独立的葡萄糖培养的帕拉斯孔斯孢酵母NRRL Y-27907培养液，以及3份独立的木糖培养的该菌株培养液中提取总RNA。每张芯片搭载362,487个探针，探针沿帕拉斯孔斯孢酵母NRRL Y-27907全基因组平铺排布，平均长度为54.5±4.0 nt，探针间中位间距为29 nt，可检测对应位点的基因表达水平。在数据处理阶段，仅保留与已注释的蛋白质编码基因对应的探针用于后续分析。