Pneumocystis jirovecii msg genes repertoires. Pneumocystis jirovecii

Characterization of the expressed and non-expressed repertoires of P. jirovecii MSG I in patients. The full-length MSG I genes linked, or not linked, to the single copy promoter will be amplified from patients samples using generic primers. The PCR products will be deep sequenced using high throughput circular consensus sequencing that generates long and highly accurate reads. We will compare the composition of the expressed and non-expressed repertoires of MSG I between patients from different geographical locations and with various underlying conditions. The results will help understanding the mechanisms of mutually exclusive expression, and thus the strategy of antigenic variation of the fungus.

针对患者体内耶氏肺孢子菌（P. jirovecii）主要表面糖蛋白I（MSG I）的表达与未表达基因谱系的特征解析。本研究将通过通用引物从患者样本中扩增与单拷贝启动子相连或不相连的全长MSG I基因；随后采用可生成长读长且高精度测序读段的高通量环形共识测序（high throughput circular consensus sequencing）技术，对聚合酶链式反应（PCR）产物开展深度测序。本研究将对比不同地理区域、伴有各类基础疾病的患者群体间，MSG I表达与未表达基因谱系的组成差异。该研究结果将有助于阐明该真菌互斥性表达的调控机制，进而揭示其抗原变异的策略。