Pseudomonas aeruginosa promotes persistence of Stenotrophomonas maltophilia via increased adherence to depolarized respiratory epithelium

In this study, we used host and bacterial RNA sequencing to evaluate the impact of dual species infections with S. maltophilia and P. aeruginosa on CF disease progression. To evaluate S. maltophilia transcript profiles we used a recently described method for selective capture of bacterial mRNA transcripts with strain specific RNA probes. RNA-sequencing of tanscriptome from mouse lung infected with Stenotrophomonas maltophilia, Pseudomonas aerugionsa, or both.

本研究通过宿主与细菌RNA测序技术，评估嗜麦芽窄食单胞菌（S. maltophilia）与铜绿假单胞菌（P. aeruginosa）双重感染对囊性纤维化（CF）疾病进展的影响。为解析嗜麦芽窄食单胞菌的转录本表达谱，我们采用了近期报道的、利用菌株特异性RNA探针选择性捕获细菌mRNA转录本的实验方法。我们对感染了嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）、铜绿假单胞菌（Pseudomonas aeruginosa），或二者共感染的小鼠肺部转录组开展了RNA测序。