Combination treatment of PTUPB with enzalutamide in C4-2B MDVR cells

To explore the gene regulatory mechanisms underlying PTUPB treatment in drug-resistant prostate cancer cells. We performed RNA sequencing analyses using PTUPB-treated C4-2B MDVR cells with or without enzalutamide treatment to identify the gene programs affected by the treatments. Comparative gene expression profiling analysis of RNA-seq data for C4-2B MDVR cells and its treatments (20 μM enzalutamide, PTUPB 10 μM to PTUPB 20 μM and their combination).

本研究旨在探究PTUPB治疗耐药性前列腺癌细胞的潜在基因调控机制。我们对经PTUPB处理、且联合或不联合恩扎卢胺（enzalutamide）处理的C4-2B MDVR细胞开展RNA测序（RNA sequencing）分析，以明确上述治疗手段所影响的基因表达程序。此外，本研究针对C4-2B MDVR细胞及其各处理组（20 μM恩扎卢胺、10 μM至20 μM PTUPB单药处理，以及二者联合给药处理）的RNA-seq数据，完成了比较性基因表达谱分析。