Effect of neutrophil depletion on gene expression im macrophages and fibroblasts from mouse lung and skin

To investigate the effect of the absence of neutrophils in the transcriptome of fibroblast and macrophage from lung and skin we injected DT to Mrp8 CRE+ iDTR and Mrp8 CRE- iDTR mice every two days for a week and isolated lung and skin fibroblasts and macrophages by cell sorting . We then performed gene expression profiling analysis using data obtained from RNA-seq of macrophages and fibroblast 7 days after DT treatment and compared the transcriptomes of macrophages and fibroblasts in control or neutrophil depletion animals. We focused on the role of neutrophils on the regulation of extracelular matrix genes in fibroblasts and macrophages and found that neutrophil depletion does not significantly affect the RNA level of matrix-related transcripts in those cells. Comparative gene expression profiling analysis of RNA-seq data for lung and skin macrophages and fibroblast from control and neutrophil depleted animals

为探究中性粒细胞（neutrophils）缺失对肺与皮肤来源成纤维细胞（fibroblast）及巨噬细胞（macrophage）转录组（transcriptome）的影响，我们每隔两天向Mrp8 CRE+ iDTR与Mrp8 CRE- iDTR小鼠注射白喉毒素（DT），持续一周，并通过细胞分选分离肺与皮肤的成纤维细胞及巨噬细胞。随后，我们利用DT处理7天后的巨噬细胞与成纤维细胞的RNA测序（RNA-seq）数据开展基因表达谱分析，并对比了对照组与中性粒细胞缺失小鼠中巨噬细胞及成纤维细胞的转录组。我们重点关注了中性粒细胞对成纤维细胞与巨噬细胞中细胞外基质（extracellular matrix）基因的调控作用，研究发现中性粒细胞缺失并未显著改变上述细胞中基质相关转录本的RNA水平。本研究针对对照组与中性粒细胞缺失小鼠的肺、皮肤来源巨噬细胞及成纤维细胞的RNA-seq数据开展了对比基因表达谱分析。