Iron-Deficiency in Pregnancy: Effects on the Rat Hippocampus

Whole-genome bisulfite sequencing on the rat hippocampus identifies DMRs near genes concerning neuronal development and plasticity which are affected by an iron-deficient diet in pregnancy. Overall design: Hippocampus samples from three iron-sufficient rats, 3 iron-deficient rats are compared with whole-genome bisulfite sequencing.

对大鼠海马体开展全基因组亚硫酸氢盐测序（Whole-genome bisulfite sequencing），成功鉴定出妊娠期间缺铁饮食所影响的、与神经元发育和可塑性相关基因邻近的差异甲基化区域（Differentially Methylated Regions，简称DMRs）。总体实验设计：选取3只铁充足大鼠与3只缺铁大鼠的海马体样本，通过全基因组亚硫酸氢盐测序进行对比分析。