The quantitation of biofilm formation by crystal violet in three day old and six day old biofilms.

A regression analysis was performed to determine whether there were any strain specific differences in the levels of biofilm formation at three days compared to the R20291reference. This was repeated for the six day old biofilms. p<0.05 indicates a significant difference from the reference R20291. The COV indicates whether the difference in biofilm formation is higher (positive number) or lower (negative number) compared to the R20291reference. A regression analysis was also used to determine whether there were significant changes in the biofilm formation from three days to six days, p<0.01.

本研究开展回归分析，以探究相较于R20291参考菌株，不同菌株在培养3天时的生物膜形成水平是否存在菌株特异性差异。该分析流程同样应用于6日龄生物膜样本的检测。当p<0.05时，表明待测菌株与参考菌株R20291之间存在显著性差异。变异系数（Coefficient of Variation, COV）可用于指示相较于参考菌株R20291，生物膜形成水平的差异方向：数值为正时代表待测菌株的生物膜形成水平更高，数值为负则代表其水平更低。本研究同时采用回归分析，以探究生物膜形成水平在培养3天至6天的周期内是否存在显著性变化，该检验的显著性阈值设为p<0.01。