Small-RNA transcriptome sequencing of individual cells

Several classes of small RNAs have been described, however to molecularly profile them require large numbers of cells. Here, we developed a method for single-cell small-RNA sequencing that we applied to naïve and primed human embryonic stem cells and to cancer cells. Single-cell profiling of microRNAs and fragments of tRNAs and snoRNAs revealed that, in particular, microRNAs have unrecognized potential to separate cell types and states. Overall design: Small-RNA transcriptome sequencing (Small-seq) is a method designed to capture all small RNAs at single cells

目前已报道了多种类型的小分子RNA（small RNAs），但对其进行分子谱型分析通常需要大量细胞。本研究开发了一种单细胞小分子RNA测序方法，并将其应用于初始态（naive）与始发态（primed）人胚胎干细胞及癌细胞的研究。通过对微RNA（microRNAs）、转运RNA（tRNA）及小核仁RNA（snoRNAs）的片段开展单细胞谱型分析后发现，尤其是微RNA，具备此前未被揭示的区分细胞类型与细胞状态的潜力。实验整体设计：小分子RNA转录组测序（Small-RNA transcriptome sequencing, Small-seq）是一种可在单细胞层面捕获所有小分子RNA的测序方法。