Microbial Community Diversity of Jinghong Laying Hens at Peak Production Based by 16S rRNA Sequencing

In this study, we evaluated the phylogenetic diversity within the duodenum (S), jejunum (K) and cecum (M) of the three different intestinal gut sections microbiota of 30-week-old Jinghong laying hens. The bacterial DNA was sequentially isolated and the V3 to V4 regions of 16S rRNA genes were amplified. The amplicons were then subjected to high-throughput sequencing to analyze the bacterial diversity of di?erent groups.本研究针对30周龄京红蛋鸡的十二指肠(duodenum)、空肠(jejunum)及盲肠(cecum)三个肠道区段的微生物群系统发育多样性进行了评估。实验过程中依次分离细菌基因组DNA，扩增16S rRNA基因的V3至V4可变区，随后对扩增产物开展高通量测序，进而分析不同组的细菌群落多样性。