DataSheet_2_Characterization of Vaginal Microbiota in Women With Recurrent Spontaneous Abortion That Can Be Modified by Drug Treatment.pdf

ObjectiveThe role of vaginal microbiota in recurrent spontaneous abortion (RSA) remains unknown. The purpose of this study was to investigate characteristics of vaginal microbiota and the effects of drug treatment on vaginal microbiota of patients with RSA.
MethodsA case-control study was performed, in which non-pregnant patients who experienced RSA were selected and divided into untreated and drug-treated groups. Drug-treated patients were subdivided into the metformin group, metformin plus aspirin group, and other drugs group. Healthy women who had live births and never experienced spontaneous abortion were enrolled in the control group. Characteristics of vaginal microbiomes of patients with RSA and healthy women and the impact of drug treatment on the microbiome was evaluated via 16S rRNA gene sequencing of the V3-V4 region using the Illumina MiSeq platform.
ResultsWomen who underwent RSA had lower microbial richness than healthy women. Compared to controls, the relative abundance of seven taxa (Megasphaera, Sneathia sanguinegens, Pseudomonas, Sphingomonas, Rhodococcus, Burkholderia- Caballeronia-Paraburkholderia, and Corynebacterium_1) in the patient’s vaginal microbiota changed significantly, which may be closely related to RSA. The composition of the vaginal microbial community in RSA patients was altered by drug treatment. Metformin combined with aspirin treatment significantly increased the relative abundance of vaginal Lactobacillus spp. in patients.
ConclusionAn altered vaginal microbiome composition might be associated with RSA, which could be modified by drug treatment. The effect of metformin combined with aspirin on vaginal Lactobacillus is worthy of attention.

研究目的
阴道菌群在复发性自然流产（recurrent spontaneous abortion, RSA）中的作用尚不明确。本研究旨在探究复发性自然流产患者的阴道菌群特征，以及药物治疗对其阴道菌群的影响。

研究方法
本研究采用病例对照研究设计，纳入曾发生复发性自然流产的非妊娠患者，分为未治疗组与药物治疗组；药物治疗组进一步细分为二甲双胍组、二甲双胍联合阿司匹林组及其他药物组。同时纳入曾活产且从未发生过自然流产的健康女性作为对照组。采用Illumina MiSeq测序平台对阴道菌群的16S rRNA基因V3-V4区进行测序，以此评估复发性自然流产患者与健康女性的阴道微生物组特征，以及药物治疗对微生物组的影响。

研究结果
复发性自然流产患者的微生物丰富度低于健康女性。与对照组相比，患者阴道菌群中7个分类单元（Megasphaera、Sneathia sanguinegens、Pseudomonas、Sphingomonas、Rhodococcus、Burkholderia-Caballeronia-Paraburkholderia及Corynebacterium_1）的相对丰度发生显著变化，这可能与复发性自然流产密切相关。药物治疗可改变复发性自然流产患者的阴道微生物群落组成；其中二甲双胍联合阿司匹林治疗可显著提升患者阴道内乳酸杆菌属（Lactobacillus）的相对丰度。

研究结论
阴道微生物组组成异常可能与复发性自然流产相关，且可通过药物治疗进行干预。二甲双胍联合阿司匹林对阴道乳酸杆菌的调控效果值得进一步关注。