Rhesus macaque aCGH

Sixteen individual rhesus macaque genomes were compared to a reference macaque genome (R354) on custom-designed sure-print 1M oligonucleotide microarray Agilent (Agilent Technologies) aCGH slide per manufacturer’s recommendations. a custom designed Agilent array-based comparative genomic hybridization (aCGH) platform, which comprises 950,843 unique 60-mer oligonucleotide probes specific to the rhesus macaque reference genome (rheMac2), to compare the genomic DNAs of 17 unrelated rhesus macaques of Indian origin to the genome of an unrelated sample from the same species.本研究采用定制化安捷伦（Agilent Technologies）SurePrint 1M寡核苷酸微阵列比较基因组杂交（array-based comparative genomic hybridization, aCGH）芯片，严格遵循制造商推荐的实验操作规范，将16只恒河猴（rhesus macaque）个体的基因组与恒河猴参考基因组R354进行比对。本次使用的定制化安捷伦aCGH平台包含950,843条针对恒河猴参考基因组rheMac2设计的特异性60聚体寡核苷酸探针，用于将17只印度来源的无亲缘关系恒河猴的基因组DNA，与同物种另一无亲缘关系个体的基因组进行比对。