Characterization of plankton communities and associated physicochemical metadata from onshore/offshore transects along the Beaufort Sea Shelf during different upwelling states in August-September 2017

DNA samples were collected by filtration of 1.3-4.4 liters (L) of seawater collected from the surface and chlorophyll maximum onto 0.2 micrometer (um) sterivex filters and frozen at sea. Samples were collected from repeat occupations of onshore-offshore transects that occurred before, during, and after wind-driven upwelling events. Following DNA extraction, 18S amplicon sequencing was used to characterize the eukaryotic plankton community (raw sequence data submitted to National Center for Biotechnology Information Sequence Read Archive, NCBI SRA). Additionally, quantitative polymerase chain reaction (qPCR) was used to quantify gene abundance of specific phytoplankton species. This dataset records the sample collection information (date, time, latitude, longitude, station id, conductivity-temperature-depth (CTD) bottle number, depth of sample collection, volume filtered, filter size), pertinent physicochemical metadata from the CTD sensors when each sample was collected (pressure, temperature, salinity, density, fluorescence), qPCR gene abundance, and the ratio of sequencing reads for individual phytoplankton species as a part of the whole phytoplankton community amplicon sequencing read dataset for each sample.

本数据集通过过滤法获取DNA样本：将1.3~4.4升（L）取自表层及叶绿素最大层的海水，经0.2微米（μm）Sterivex滤膜过滤后，在采样船上进行冷冻保存。样本采集自重复布设的近岸-离岸断面站位，覆盖风驱动上升流发生前、发生过程中及发生后的完整时段。完成DNA提取后，采用18S扩增子测序对真核浮游生物群落进行物种表征，原始测序数据已提交至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）序列读取存档库（Sequence Read Archive, SRA）。此外，采用定量聚合酶链式反应（quantitative polymerase chain reaction, qPCR）对特定浮游植物物种的基因丰度进行定量分析。本数据集记录了样本采集相关信息（采集日期、时间、纬度、经度、站位编号、温盐深（conductivity-temperature-depth, CTD）采水瓶编号、样本采集深度、过滤体积、滤膜孔径），以及各样本采集时由CTD传感器获取的相关理化元数据（压力、温度、盐度、密度、荧光强度）、qPCR基因丰度数据，同时还包含各样本浮游植物群落扩增子测序读段数据集中，单个浮游植物物种的测序读段占比信息。