Osteopontin Deficiency Amerliorates Alport Kindey Pathology

Kidneys were snap frozen from 2 month old wild type, Col4a3-/-, or Col4a3-/-OPN-/- mice. RNA was isolated using Mirvana Paris kit. Kidneys were snap frozen from 2 month old wild type (n=2), Col4a3-/- (n=3), or Col4a3-/-OPN-/- (n=3) mice. RNA was isolated using Mirvana Paris kit.

2月龄野生型、Col4a3⁻/⁻及Col4a3⁻/⁻OPN⁻/⁻小鼠的肾脏组织经速冻处理；采用Mirvana Paris 试剂盒（Mirvana Paris kit）分离总RNA。2月龄野生型（n=2）、Col4a3⁻/⁻（n=3）及Col4a3⁻/⁻OPN⁻/⁻（n=3）小鼠的肾脏组织经速冻处理；总RNA采用Mirvana Paris 试剂盒分离。