Whole genome transcriptomic experiments in mice heart development system

In order to understand genes expressed during posterior second heart field (pSHF) development, we performed microdissections of the region for transcriptomic analysis at mouse embryonic day 9.5. Overall design: RNA-sequencing of microdissected posterior second heart field samples at mouse embryonic day 9.5 from 5 wildtype samples.为了探究在后第二心区（posterior second heart field, pSHF）发育过程中表达的基因，我们于小鼠胚胎发育第9.5天对该区域实施显微解剖，以开展转录组学分析。整体实验设计：对5份来自野生型小鼠的胚胎第9.5天显微解剖获取的后第二心区样本进行RNA测序。