Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c9_h1930002_MTG

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snm3C-seq to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，对于解析其多样功能与复杂动物行为具有关键意义。本研究对人类大脑多个脑区开展单细胞核甲基化测序（single nucleus methylation sequencing），成功鉴定出多个独特的细胞簇，并构建层级分类系统对其进行归类。所鉴定出的细胞簇涵盖了已报道的主要脑细胞类型以及潜在亚型。我们依托本研究产生的测序数据，鉴定了各细胞簇的表观基因组特征，并明确了每个细胞簇对应的特异性调控元件。实验整体设计：对脑区实施单细胞核3C测序（snm3C-seq），以解析成年人类大脑的表观基因组图谱。