Comparison of cCENH3-ChIP experiments using different Ab amounts and washing conditions.

ChIP experiments were performed using different amounts of cCENH3 antibody (5 and 8 μg) and different washing conditions (weak wash with commercial washing buffers and strong wash with low/high salt buffers). The ChIP/Input ratio for the top 100 WGS clusters was compared between different ChIP experiments. (XLSX)染色质免疫共沉淀（Chromatin Immunoprecipitation，ChIP）实验采用了不同剂量的cCENH3抗体（5 μg与8 μg），并设置了两类洗涤条件：以商品化洗涤缓冲液进行的弱洗涤，以及以低盐/高盐缓冲液开展的强洗涤。针对前100个全基因组测序（Whole Genome Sequencing，WGS）聚类区域的ChIP/Input比值，本数据集对不同ChIP实验的结果进行了比较。（数据格式为XLSX）