2016_Koff

We recently developed a technology that allows to precisely determine the T cell receptor (TCR) ? ligand binding strength by reversibel MHC multimer staining and subsequent measurement of TCR-ligand dissociation rates via real time fluorescence microscopy. Unfortunately, this technology is very laborious and difficult to transfer to other laboratories. Therefore, we tested whether the basic principle of Koff rate measurements could be transferred to a flow cytometry-based acquisition platform.本团队近期开发了一项技术，可通过可逆主要组织相容性复合体（Major Histocompatibility Complex, MHC）多聚体染色，结合后续借助实时荧光显微镜开展的T细胞受体（T cell receptor, TCR）与配体解离速率检测，精准测定二者的结合强度。遗憾的是，该技术操作繁琐且难以向其他实验室推广转移。因此，本团队测试了能否将解离速率（Koff）测量的基本原理，迁移至基于流式细胞术（flow cytometry）的采集平台。