The Influence of Different High Fidelity DNA Polymerase on 16S rRNA Sequencing

Assessing differences due to high fidelity DNA polymerase to downstream 16S rRNA gene survey results. Variations in number of OTUs and chimera prevalence were found to be high fidelity DNA polymerase dependent.本研究旨在评估高保真DNA聚合酶（high fidelity DNA polymerase）对下游16S rRNA基因调查结果所造成的差异。研究发现，操作分类单元（Operational Taxonomic Units, OTUs）的数量与嵌合序列检出率的变化均依赖于所使用的高保真DNA聚合酶。