Data from: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay

A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a-like-b, tnfrsf5, and tnfrsf9. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and the Flavobacterium psychrophilum load are reported. These data are an extension of information presented and discussed in the article "Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)" (Kutyrev et al., 2016). Resources in this dataset:Resource Title: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay. File Name: Web Page, url: https://www.sciencedirect.com/science/article/pii/S2352340917300331 Data in Brief data article providing expression values for a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Data are available for download as Excel spreadsheet.Resource Software Recommended: Microsoft Excel,url: https://office.microsoft.com/excel/

本研究开发并优化了一种GeXP多重RT-PCR检测方法，该法可同步测量彩虹 trout (Oncorhynchus mykiss) 中一系列与免疫相关的基因表达，特别聚焦于肿瘤坏死因子及其配体/受体系统以及急性期反应基因。数据集涵盖了drpt、il11a、il1b1、il1b2、il1b3、il1r-like-1(e3-5)、il1r-like-1(e9-11)、il1r1-like-a、il1r1-like-b、il1r2、saa、tnfa1、tnfa2、tnfa3、tnfrsf1a、tnfrsf1a-like-a、tnfrsf1a-like-b、tnfrsf5以及tnfrsf9等基因的表达值。基因表达在彩虹 trout 的抗病系（ARS-Fp-R）和易感系（ARS-Fp-S）的挑战后四个时间点进行测量。此外，还报告了鱼体重量、脾指数以及弗劳地氏菌的载量。这些数据是对Kutyrev等人（2016年）在文章“对弗劳地氏菌的挑战后，在抗病和易感系彩虹 trout 中检测到的促炎细胞因子和细胞因子受体基因表达动力学”中呈现和讨论的信息的扩展。数据集中包含的资源：
资源标题：使用新型GeXP多重RT-PCR检测法测量的彩虹 trout (Oncorhynchus mykiss) 促炎细胞因子和细胞因子受体基因表达数据集。
文件名：网页，url：https://www.sciencedirect.com/science/article/pii/S2352340917300331
数据简报数据文章，提供了彩虹 trout (Oncorhynchus mykiss) 中一系列与免疫相关的基因表达值，重点集中在肿瘤坏死因子及其配体/受体系统以及急性期反应基因。数据可作为Excel电子表格下载。
推荐资源软件：Microsoft Excel，url：https://office.microsoft.com/excel/