Epigenome analysis of normal and cervical cancer with human papilloma virus type 16 or 18 positive. Epigenome analysis of normal and cervical cancer with human papilloma virus type 16 or 18 positive

The Illumina 850k Human DNA methylation EPIC BeadChip was used to obtain DNA methylation profiles across approximately 850,000 CpGs in the formalin-fixed and paraffin-embedded tissue sections(FFPE). Samples included 3 normal samples without HPV and 6 cervical cancer samples (three HPV 16 positive,three HPV 18 positive). Overall design: Bisulphite converted DNA from the 9 samples were hybridised to The Illumina 850k Human DNA methylation EPIC BeadChip

本研究采用Illumina 850K人类DNA甲基化EPIC微珠芯片（Illumina 850k Human DNA methylation EPIC BeadChip），对福尔马林固定石蜡包埋组织切片（FFPE）中约85万个CpG位点的DNA甲基化图谱进行获取。本次实验样本包含3例HPV阴性的正常样本与6例宫颈癌样本（其中3例为HPV16阳性，3例为HPV18阳性）。实验整体设计如下：将来自这9份样本的亚硫酸氢盐转化DNA，与上述Illumina 850K人类DNA甲基化EPIC微珠芯片进行杂交。