Exposure of rat to a variety of toxicants, bone marrow assayed by CodeLink microarray. Rattus norvegicus

DrugMatrix is a comprehensive rat toxicogenomics database and analysis tool developed to facilitate the integration of toxicogenomics into hazard assessment. Using the whole genome and a diverse set of compounds allows a comprehensive view of most pharmacological and toxicological questions and is applicable to other situations such as disease and development. Overall design: Complete Drug Matrix dataset for rat bone marrow. Approximately 600 different compounds were profiled in up to 8 different rat tissues by obtaining tissue samples from test compound-treated and vehicle control-treated rats in biological triplicates for gene expression analysis after 0.25, 1, 3, and 5 days of exposure with daily dosing. In a few studies (1.8%), 7 days of exposure was substituted for 5 days of exposure. Samples were hybridized to CodeLink RU1 10K rat arrays (Amersham Biosciences).

DrugMatrix是一款综合性的大鼠毒理基因组学（toxicogenomics）数据库与分析工具，旨在推动毒理基因组学整合入危险度评估流程。依托全基因组覆盖与多样化的化合物库，该工具可对绝大多数药理学与毒理学问题提供全景式解析，且可推广应用于疾病、发育生物学等其他研究场景。
整体实验设计：本次数据集为大鼠骨髓来源的完整DrugMatrix数据集。研究共纳入约600种不同化合物，通过对受试化合物处理组与赋形剂对照组大鼠采集组织样本（每组设3次生物学重复），在每日给药的前提下，分别于暴露0.25、1、3及5天后取样开展基因表达分析，检测组织最多覆盖8种不同的大鼠组织。在占比1.8%的少量研究中，以7天暴露时长替代了5天暴露时长。所有样本均与CodeLink RU1 10K大鼠基因芯片（Amersham Biosciences）进行杂交。