Interactome of the two T. brucei Poly(A) binding protein paralogs PABP1and PABP2

Poly(A) binding proteins (PABPs) regulate mRNA fate by controlling mRNA stability and translation through interactions with both the poly(A) tail and eIF4F complex. Here we identified a protein interaction cohort for both T. brucei PABPs by cryo-mill/affinity purification-mass spectrometry.

聚腺苷酸结合蛋白（Poly(A) binding proteins，PABPs）可通过与多聚腺苷酸尾（poly(A) tail）以及真核翻译起始因子4F复合物（eIF4F complex）相互作用，调控mRNA的稳定性与翻译过程，进而调控mRNA的命运。本研究借助冷冻研磨/亲和纯化-质谱联用技术（cryo-mill/affinity purification-mass spectrometry），鉴定了布氏锥虫（Trypanosoma brucei，T. brucei）的两种PABPs的蛋白质相互作用组群。