RNA-sequencing of Candida albicans RAP1-deletion mutant

The goal is to explore the functions of the transcription factor Rap1 in Candida albicans. The wild-type strain (SC5314) and the RAP1-deletion mutant strain were cultured in YPD medium with 400 micromolar of iron chelator bathophenanthrolinedisulfonic acid for 24 h, and subsequently cultured in YPD medium for 5h. Cell were then collected to assess the differences in the gene expression.本研究旨在探究白色念珠菌（Candida albicans）中转录因子（transcription factor）Rap1的功能。将野生型菌株（SC5314）与RAP1缺失突变菌株接种于添加了400微摩尔铁螯合剂浴铜灵二磺酸（bathophenanthrolinedisulfonic acid）的YPD培养基中培养24小时，随后转接至YPD培养基中继续培养5小时。收集细胞以分析其基因表达差异。