Annotation file for de novo assembled transcriptome of hybrids between Epidendrum fulgens and E. puniceoluteum (Orchidaceae)

We used Blast2GO to perform a functional annotation of the de novo assembled transcriptome of hybrids between Epidendrum fulgens and E. puniceoluteum. To do so, we filtered for contigs longer than 300 bp and then aligned the contigs against the NCBI non-redundant NR protein database with an e-value cut-off of 10e−3 using the CloudBlast service. Simultaneously, we used the InterProScan tool to retrieve protein domains and motif information. Lastly, we processed all matching transcripts and assigned gene ontology (GO) terms following the Blast2GO annotation workflow.

本研究采用Blast2GO对Epidendrum fulgens与E. puniceoluteum杂交种的从头组装转录组（de novo assembled transcriptome）开展功能注释。为此，我们首先筛选出长度大于300 bp的重叠群（contigs），随后通过CloudBlast服务将重叠群比对至NCBI非冗余（NR）蛋白质数据库，设置e值阈值为10×10^-3；同时，借助InterProScan工具获取蛋白质结构域与基序信息；最后，按照Blast2GO注释流程对所有匹配的转录本进行处理，并为其赋予基因本体（GO）术语。