Ileal mucosa bacterial amplicons of starter pigs fed high and low complex diets, with and without antibiotics nursery diets. Genome sequencing

Weaned pigs were fed HIGH or LOW complexity diets, with or without antibiotics, for 6 weeks, followed by a common grower diet. Pigs were killed at 2 (n = 6) and 8 (n = 6) weeks post-weaning (periods of reduced and improved growth, respectively). Mucosal bacteria were removed; DNA was extracted and amplified using the V1-V3 region of the 16S rRNA gene.

断奶仔猪被饲喂高复杂度日粮与低复杂度日粮，分别添加或不添加抗生素，持续6周，随后更换为常规生长日粮。分别于断奶后2周（n=6）与断奶后8周（n=6）处死仔猪，两个时间点分别对应生长受限与生长改善的实验阶段。分离得到黏膜细菌后，提取其DNA，并以16S rRNA基因（16S ribosomal RNA gene）的V1-V3可变区作为扩增靶区域进行扩增。