RNA-sequencing in LNCaP prostate cancer cells. RNA-sequencing in LNCaP prostate cancer cells

We used RNA-Seq to profile gene expression in LNCaP cells with and without PRMT1 knockdown. Overall design: RNA-Seq of LNCaP cells infected with doxycycline-inducible PRMT1 shRNA and were either untreated (control) or treated with doxycycline to induce PRMT1 knockdown. Three replicates for each condition.

我们采用RNA测序（RNA-Seq）技术对存在PRMT1基因敲低与未进行PRMT1基因敲低的LNCaP细胞开展基因表达谱分析。实验整体设计如下：将感染多西环素（doxycycline）诱导型PRMT1短发夹RNA（small hairpin RNA, shRNA）的LNCaP细胞分为两组，一组不予任何处理（对照组），另一组经多西环素处理以诱导PRMT1基因敲低；每组设置3次生物学重复。