Xiphophorus maculatus Genome Sequencing

The platyfish DNA for shotgun sequencing, and for BAC libraries, is derived from a single female (Xiphophorus maculates, Strain - JP 163 A, sample id: XMAC-090115_JP163A) from the laboratory of Dr. Ron Walter, Texas State University, San Marcos, Texas, USA. Fish have been mated and given rise to generation 105. Two brother-sister matings of the 105 generation have dropped broods from which the female was chosen for sequencing. These fish are bred and maintained at the: Xiphophorus Genetic Stock Center, Texas State University, San Marcos, Texas.

用于鸟枪法测序与BAC文库构建的斑剑尾鱼（Xiphophorus maculatus）DNA，提取自美国德克萨斯州圣马科斯市德克萨斯州立大学Ron Walter博士实验室的单只雌性个体，该个体所属品系为JP 163 A，样本编号为XMAC-090115_JP163A。该种群经多代交配繁育，已培育至第105代。研究人员对第105代个体开展两次兄妹交配，获得多窝幼鱼，并从中选取了用于测序的雌性个体。上述繁育种群均饲养于美国德克萨斯州圣马科斯市德克萨斯州立大学剑尾鱼遗传资源中心（Xiphophorus Genetic Stock Center）。