Effect of short-term prescription opioids on DNA methylation of the OPRM1 promoter. Effect of short-term prescription opioids on DNA methylation of the OPRM1 promoter

Evaluate the effect of short-term use of precribed opioids on DNA methylation Overall design: Genome-wide DNA methylation was assayed in a cohort of opioid-naïve participants who underwent standard dental surgery followed by opioid self-administration. Saliva samples were collected longitudinally before surgery (visit 1), and at two postsurgery visits at 2.7 ± 1.5 days (visit 2), and 39 ± 10 days (visit 3) after the discontinuation of opioid analgesics.

评估处方阿片类药物短期使用对DNA甲基化(DNA methylation)的影响
试验整体设计：对接受标准牙科手术并随后自行使用处方阿片类药物的阿片类药物初治(opioid-naïve)队列参与者开展全基因组DNA甲基化检测。
研究人员纵向采集唾液样本，采样时间点分别为术前（访视1）、术后访视2（停用阿片类镇痛药物后2.7±1.5天）以及术后访视3（停用阿片类镇痛药物后39±10天）。