The Glucocorticoid Receptor couples endocrine signalling to synaptic activity dependent gene transcription via a metabotropic glutamate receptor-regulated pathway in zebrafish - BisPCR2 data. The Glucocorticoid Receptor couples endocrine signalling to synaptic activity dependent gene transcription via a metabotropic glutamate receptor-regulated pathway in zebrafish - BisPCR2 data

The Glucocorticoid Receptor (GR) co-ordinates metabolic and behavioural responses to stressors. We hypothesised that GR influences behaviour by modulating epigenetic and transcriptional processes in the brain. Using the zebrafish as a model organism, the brain methylomes of wild-type and grs357 mutant adults were analysed and GR-sensitive, differentially methylated regions (GR-DMRs) were identified. DMRs linked to genes with biologically salient functions were selected for confirmatory deep sequencing analysis in wild-type and GR mutant 5dpf larval heads, using the BisPCR2 technique (Bernstein et al., 2015). Our results demonstrate that the genes fkbp5 and aplp1 exemplify two distinct modes of GR-regulated, glucocorticoid-responsive DNA methylation in the brain. Overall design: 96 larval head BisPCR2 samples (24 GRWT, 24 GRWT-BM, 24 GRmut, 24 GRmut-BM)

糖皮质激素受体（Glucocorticoid Receptor, GR）可协调机体对应激源的代谢与行为应答。本研究提出假说：GR通过调控大脑中的表观遗传与转录过程来影响行为。我们以斑马鱼为模式生物，对野生型与grs357突变型成年个体的大脑甲基化组开展分析，鉴定出GR敏感型差异甲基化区域（GR-sensitive Differentially Methylated Regions, GR-DMRs）。针对与具有关键生物学功能的基因相关的DMRs，我们采用BisPCR2技术（Bernstein等，2015），对野生型及GR突变型5日龄幼鱼头进行验证性深度测序分析。本研究结果表明，fkbp5与aplp1基因分别代表了大脑中GR调控的、糖皮质激素响应性DNA甲基化的两种不同模式。实验整体设计：96份幼鱼头BisPCR2样本（24份GR野生型组、24份GR野生型-BM组、24份GR突变型组、24份GR突变型-BM组）