Effects of doxorubicin on miRNA expression in tumor cell small extracellular vesicles

To examine the changes of miRNA in the small extracellular vesicles of breast cancer cells receiving DOX chemotherapy. Human breast cancer cells (MDA-MB-231 cells) were cultured with exorcism-free serum, and treated with DOX. After 24h, cell supernatant was collected and small extracellular vesicles were extracted by ultra-fast centrifugation method, and miRNA content in small extracellular vesicles was obtained for high-throughput miRNA sequencing. miRNA sequencing of 6 samples of small extracellular vesicles from DOX and no drug (control) -treated human breast cancer cells.

本数据集旨在探究接受多柔比星（DOX）化疗的乳腺癌细胞来源的小型细胞外囊泡（small extracellular vesicles）内microRNA (miRNA)的表达变化。采用无外泌体血清培养人乳腺癌细胞（MDA-MB-231细胞），并以DOX进行处理。培养24小时后收集细胞上清液，通过超速离心法提取小型细胞外囊泡，随后开展高通量miRNA测序以检测小型细胞外囊泡内的miRNA含量。本数据集包含经DOX处理与未给药（对照组）处理的人乳腺癌细胞来源的6份小型细胞外囊泡样本的miRNA测序数据。