10X genomics libraries for scRNA-seq of zebrafish embryos (2dpf) treated with uncoated and oligolysine-PEG copolymer coated DNA origami wireframe nanosheets. 10X genomics libraries for scRNA-seq of zebrafish embryos (2dpf) treated with uncoated and oligolysine-PEG copolymer coated DNA origami wireframe nanosheets

To study the biodistribution of DNA origami in zebrafish and to determine differences in biodistribution based on the coating of the nanostructure using oligolysine-PEG copolymers, zebrafish embryos (2dpf) were injected with coated or uncoated DNA origami (wireframe nanosheets) without any targeting molecule, but containing a detection fluorophores. After treatment, the embryos were disassociated into a single-cell suspension and sorted through FACS for embryos containing nanostructures (fluorophores). 10X sc-libraries were generated from the sorted embryos and sequenced to establish cell type predictions for cells with coated and uncoated structures respectively.

为研究DNA折纸（DNA origami）在斑马鱼体内的生物分布，并明确基于寡赖氨酸-聚乙二醇共聚物包覆的纳米结构所引发的生物分布差异，本研究向受精后2天（2 days post fertilization, 2dpf）的斑马鱼胚胎注射了无任何靶向分子但带有检测用荧光基团的包覆型或非包覆型DNA折纸（线框纳米片）。处理完成后，将胚胎解离为单细胞悬液，通过荧光激活细胞分选（FACS）筛选出携带纳米结构（即携带荧光基团）的胚胎。随后从筛选得到的胚胎中构建10X单细胞文库并进行测序，以分别针对带有包覆型纳米结构与非包覆型纳米结构的细胞建立细胞类型预测模型。