Transcriptome of WT, Dgcr8_KO, Drosha_KO, and Ago1&2_double KO mouse embryonic stem cells cultured in 2i. Transcriptome of WT, Dgcr8_KO, Drosha_KO, and Ago1&2_double KO mouse embryonic stem cells cultured in 2i

Purpose: The goals of this study was to assess the impact of different RNAi-mutant proteins on mESC transcriptome in 2i condition. Overall design: mRNA profiles of different RNAi-mutant mouse embryonic stem cells (E14) were generated by deep sequencing, in duplicate, using Illumina HiSeq2000.

研究目的：本研究旨在评估2i培养条件下，不同RNA干扰（RNA interference, RNAi）突变蛋白对小鼠胚胎干细胞（mouse embryonic stem cells, mESC）转录组的影响。
实验设计：采用Illumina HiSeq2000平台开展高通量测序，对不同RNAi突变型小鼠胚胎干细胞（E14细胞系）的mRNA表达谱进行双重复检测。