BETA-FOR_SP4_Nematodes_2023_Individuals

CAUTION! Difference to other nematode dataset (BETA-FOR_SP4_Nematodes_2023): This dataset contains the ABSOLUTE identified nematode individuals per patch and taxa. The other dataset contains the respective number of individuals per g of dry soil. Use THIS dataset if you use iNEXT, as you need singletons to do so. Methods: We took four soil samples per patch (5cm diameter, 10 cm depth) within 30 cm radius from TOMST logger after roughly removing the litter. We pooled these samples per patch, sieved the soil (2mm) and extracted nematodes as soon as possible (nematodes need to be alive for this method) with the Baermann-Funnel Method. To do so, we used ~20 g fresh soil. After extraction, nematoes were fixed in 4% formalin, counted and if possible 100 individuals (10% if more than 1000 in the sample) morphologically determined. As some of the nematode samples had lots of debris besides the nematodes, it was not possible to identify 100 specimens in each sample. We advise caution when interpreting the data; while standardizing approaches like iNEXT are possible, we do not recommend analyzing nematode indices or trophic groups due to the varying number of identified individuals.注意！本数据集与另一线虫数据集（BETA-FOR_SP4_Nematodes_2023）存在差异：本数据集记录的是每个样区（patch）内各分类单元（taxa）的已鉴定线虫个体绝对数量，而另一数据集记录的是每克干土中的对应线虫个体数。若需使用iNEXT进行分析，需使用单例（singletons）数据，因此请选用本数据集。 研究方法：在粗略移除地表枯落物后，于TOMST环境记录仪（TOMST logger）周边30 cm半径范围内，采集4个直径5 cm、深度10 cm的土样作为一个样区的样本。将同一样区的土样混合均匀，将土壤过2 mm孔径筛，随后尽快采用贝尔曼漏斗法（Baermann-Funnel Method）提取线虫，该方法要求线虫保持活体状态，每份土样使用约20 g新鲜土壤。提取完成后，将线虫置于4%福尔马林中固定，进行计数，若样本中线虫数量不超过1000，则尽可能完成100个个体的形态学鉴定；若样本中线虫数量超过1000，则完成总个体数10%的形态学鉴定。 由于部分线虫样本中除线虫外还伴随大量碎屑杂质，并非所有样本均可完成100个个体的鉴定。我们提醒在解读本数据时需谨慎：尽管可采用iNEXT等标准化分析方法，但鉴于不同样本的已鉴定个体数量存在差异，我们不建议基于本数据集开展线虫指数或营养类群的相关分析。