Histone modifications (H3K4me1, H3K4me3, and H3K27ac) in IGF-2-preprogrammed macrophages and control macrophages

Macrophages with innate immune memory are in a modified steady-status with altered responsiveness to stimulation featured by rewired epigenetic program. To better convey the concept of IGF-2-mediated innate immune memory and help understanding the anti-inflammatory responsiveness of macrophages, we analyzed histone modifications (H3K4me1, H3K4me3, and H3K27ac) by whole genome chip-sequencing analysis in control macrophages and IGF-2-preprogrammed macrophages. In the whole genome chip-sequencing analysis, we found that IGF-2 has limited effects on H3K4me1and H3K4me3, but mainly modulates H3K27ac status during macrophages maturation. Overall design: Examination of 3 different histone modifications in cotrol macrophages and IGF-2-preprogrammed macrophages

带有固有免疫记忆的巨噬细胞处于修饰后的稳态，其对刺激的应答发生改变，核心特征为表观遗传程序的重塑。为更好地阐释IGF-2介导的固有免疫记忆这一概念，并助力理解巨噬细胞的抗炎应答特性，我们采用全基因组染色质免疫共沉淀测序（ChIP-sequencing）技术，对对照组巨噬细胞与IGF-2预编程巨噬细胞中的组蛋白修饰（H3K4me1、H3K4me3及H3K27ac）进行了分析。在本次全基因组ChIP测序分析中，我们发现IGF-2对H3K4me1与H3K4me3的调控作用较为有限，但其在巨噬细胞成熟过程中主要调控H3K27ac的修饰状态。整体实验设计：对对照组巨噬细胞与IGF-2预编程巨噬细胞的3种不同组蛋白修饰进行检测。