Anomaly Detection and Removal Strategies for In-line Permittivity Sensor Signal Used in Bioprocesses

Dataset from 8 recombinant yeast P. pastoris laboratory bioreactor fermentations producing Hepatitis B surface (HBsAg) antigen (exps. 1-4) and Hepatitis B core (HBcAg) antigen. An in-situ permittivity probe (Hamilton, Incyte) was used in these experiments for permittivity and conductivity data logging. The dataset consists of the following values: Fermentation time, minutes; Stirrer speed, RPM; Substrate (50% Glycerol/100% Methanol) feed rate, mL/min; Base (25% NH4OH) pump volume, mL; Antifoam (100% Sigma 204) pump volume, mL; Substrate (50% Glycerol/100% Methanol) feed pump volume, mL; Culture medium conductivity, mS/cm; Culture medium permittivity, pF/cm; Permittivity at 17 scanning frequencies (300-10,000 kHz), pF/cm. Dataset has been generated within the framework of a postdoctoral project (ERDF and Latvian state-funded research application grant No. 1.1.1.2/VIAA/1/16/186). Dataset has been used in the following articles: Grigs, O., Bolmanis, E., & Galvanauskas, V. (2021). Application of In-Situ and Soft-Sensors for Estimation of Recombinant P. pastoris GS115 Biomass Concentration: A Case Analysis of HBcAg (Mut+) and HBsAg (MutS) Production Processes under Varying Conditions. Sensors, 21(4), 1268. https://doi.org/10.3390/s21041268 Bolmanis, E., Ramm, S., Pein-Hackelbusch, M., Galvanauskas, V., Grigs, O. Anomaly Detection and Removal Strategies for In-line Permittivity Sensor Signal Used in Bioprocesses. Front. bioeng. biotechnol.本数据集源自8批重组巴斯德毕赤酵母（Pichia pastoris，P. pastoris）实验室生物反应器发酵实验，其中实验1-4用于生产乙型肝炎表面抗原（Hepatitis B surface antigen, HBsAg），其余4批用于生产乙型肝炎核心抗原（Hepatitis B core antigen, HBcAg）。本实验采用汉密尔顿（Hamilton）Incyte型原位介电常数探头（in-situ permittivity probe），用于采集介电常数与电导率的数据日志。 本数据集包含以下参数： 1. 发酵时长，单位：分钟； 2. 搅拌转速，单位：转每分钟（RPM）； 3. 底物（50%甘油/100%甲醇）进料速率，单位：毫升每分钟（mL/min）； 4. 碱液（25%氢氧化铵）泵送体积，单位：毫升（mL）； 5. 消泡剂（100% Sigma 204）泵送体积，单位：毫升（mL）； 6. 底物（50%甘油/100%甲醇）进料泵送体积，单位：毫升（mL）； 7. 培养基电导率，单位：毫西门子每厘米（mS/cm）； 8. 培养基介电常数，单位：皮法每厘米（pF/cm）； 9. 17个扫描频率（300~10000 kHz）下的介电常数，单位：皮法每厘米（pF/cm）。 本数据集系在一项博士后研究项目框架下生成，该项目获欧洲区域发展基金（ERDF）与拉脱维亚国家资助的研究申请项目（编号1.1.1.2/VIAA/1/16/186）支持。 本数据集已应用于以下学术论文： 1. Grigs, O., Bolmanis, E., & Galvanauskas, V. (2021). 原位传感器（in-situ sensors）与软传感器（soft-sensors）在重组巴斯德毕赤酵母GS115生物质浓度估算中的应用：不同工况下HBcAg（Mut+）与HBsAg（MutS）生产过程的案例分析. Sensors, 21(4), 1268. https://doi.org/10.3390/s21041268 2. Bolmanis, E., Ramm, S., Pein-Hackelbusch, M., Galvanauskas, V., Grigs, O. 生物过程中在线介电常数传感器（in-line Permittivity Sensor）信号的异常检测与去除策略. Frontiers in Bioengineering and Biotechnology.