Changes in physiological activities and biochemical components of marine zooplankton after capture (Great Barrier Reef)

Specimens of the decapod shrimp, Acetes sibogae australis, were collected along the jetty of the Australian Institute of Marine Science (AIMS) and specimens of the copepod, Acartia australis, were collected from Davies Reef lagoon. The copepods were transported in a 20 litre bucket to the AIMS laboratory within six hours. In the laboratory each species was transferred to gently aerated aquaria containing unfiltered sea water collected from the same site as the animals and placed in a constant temperature room (24-25°C).Physiological activities and biochemical components were measured for subsamples of Acetes sibogae australis at 2, 14, 26 and 50 hours after capture. Measurements for subsamples of Acartia australis were made at 6, 14, 26 and 49 hours after capture. Respiration and excretion of ammonia and phosphate were measured using a water bottle method. Twelve replicate bottles containing Acetes sibogae australis were incubated for 2.5-3.5 hours, while 8 replicates bottles containing Acartia australis were incubated for 3.5-4.5 hours. At the end of incubation, separate water samples were siphoned off dissolved oxygen analysis and for ammonia and phosphate analyses.Animals from the incubation bottles were filtered onto GF/C filters and frozen in liquid nitrogen and stored in a freezer until analysed within 24 hours. Half of the filters from each experiment were homogenized in trichloroacetic acid for extraction of adenine nucleotides. ATP was determined by the luciferase-luminescence method, and ADP and AMP by the same method after enzymatic conversion to ATP. The remaining GF/C filters were homogenized in ETS-B solution, and these extracts were used to determine electron transport system (ETS) activity and the concentrations of protein and RNA. Laboratory studies were undertaken to investigate the decline in physiological activities, including respiration and excretion rates, of two species of marine zooplankton after capture. The results were used to assess whether capture stress or shortage of food contributed to declines. These experiments were a component of the project "The effect of laboratory conditions on the extrapolation of experimental measurements to the ecology of marine zooplankton".

本数据集的样本包括：于澳大利亚海洋科学研究所（Australian Institute of Marine Science, AIMS）栈桥沿岸采集的十足目虾类（decapod shrimp）西宝莹虾澳洲亚种（Acetes sibogae australis），以及采自戴维斯礁泻湖（Davies Reef lagoon）的桡足类（copepod）澳氏纺锤水蚤（Acartia australis）。澳氏纺锤水蚤样本以20升桶装载，于6小时内转运至AIMS实验室。抵达实验室后，两个物种均被转移至装有采自原采集地未过滤海水的轻度充气水族箱，并置于恒温室（24~25℃）中暂养。研究人员分别于捕获后2、14、26及50小时，对西宝莹虾澳洲亚种的子样本开展生理活性与生化组分测定；针对澳氏纺锤水蚤的子样本测定则于捕获后6、14、26及49小时进行。氨、磷酸盐的排泄量与呼吸耗氧率采用培养瓶法测定：西宝莹虾澳洲亚种设置12个重复培养瓶，培养时长为2.5~3.5小时；澳氏纺锤水蚤设置8个重复培养瓶，培养时长为3.5~4.5小时。培养结束后，分别虹吸取水样用于溶解氧、氨氮及磷酸盐含量分析。将培养瓶中的浮游动物样本通过GF/C滤膜过滤后，置于液氮中冷冻保存，并于24小时内完成后续分析。每个实验的半数滤膜用三氯乙酸（trichloroacetic acid）匀浆以提取腺嘌呤核苷酸（adenine nucleotides）：三磷酸腺苷（ATP）采用荧光素酶发光法（luciferase-luminescence method）测定，二磷酸腺苷（ADP）与一磷酸腺苷（AMP）经酶促转化为ATP后，采用相同方法完成测定。剩余GF/C滤膜用ETS-B溶液（ETS-B solution）匀浆，所得提取物用于测定电子传递系统（ETS）活性以及蛋白质与RNA含量。本研究通过实验室实验，探究了两种海洋浮游动物（zooplankton）捕获后生理活性（包括呼吸与排泄速率）的下降趋势，以评估捕获应激或食物匮乏是否为导致活性下降的诱因。本实验为项目"实验室条件对实验测量结果外推至海洋浮游动物生态学的影响"的组成部分。