Fiber Photometry Recording of Dopamine Dynamics and Population-Level Ca2+ Activity in the Mouse Forelimb Motor Cortex

This dataset was collected during the implementation of the project ‘Real-time imaging of dopamine release and neuronal populations dynamics in the motor cortex of awake mice – decoding of reward-related signals and movement parameters’ funded by the National Science Centre, Poland 2020/39/D/NZ4/00503. In the project, head-fixed D1Cre and D2Cre mice were trained to perform skilled forelimb movements with a joystick to collect water rewards. Fiber photometry was used to simultaneously monitor dopamine dynamics and population-level calcium activity from D1+ and D2+ neurons in the forelimb motor cortex during behavior.

本数据集采集自波兰国家科学中心（National Science Centre, Poland）资助项目编号为2020/39/D/NZ4/00503的《清醒小鼠运动皮层多巴胺释放与神经元群体动态实时成像——奖赏相关信号与运动参数解码》的实施过程中。该项目中，研究人员对头部固定的D1Cre与D2Cre小鼠进行训练，使其可通过操纵操纵杆完成熟练前肢运动以获取水奖赏。实验期间，研究者采用光纤光度法（fiber photometry）同步监测前肢运动皮层内D1阳性与D2阳性神经元的多巴胺动态及群体水平钙活动。