Allocation of littermates to experimental tests

Each dam provided four male mice to the study. Littermates were tested according to the schedule reported in the table. E.g. mouse 2 performed the novelty-seeking test between PND 37-41 and was then assessed for immune response between PND 90-104. This schedule was adopted in order to maximize the information obtained by each mouse and to minimize the potential carry-over effects due to previous testing. In one case we could not use naïve mice; therefore we first exposed mice to the least stressful procedure (novelty seeking) and then waited a long time before the second test (immune resistance).每只母鼠为本研究提供四只雄性小鼠。同窝仔鼠按照附表所列流程接受测试。例如，2号小鼠于出生后第37至41天（Postnatal Day，PND 37-41）完成新奇探索测试，随后在出生后第90至104天（PND 90-104）接受免疫应答评估。本测试流程的设计旨在最大化单只小鼠可获取的实验数据，同时最大限度削弱既往测试所引发的潜在遗留效应。有一例实验未能使用未经过任何实验处理的naïve小鼠（naïve mice）；因此我们先让小鼠接受应激程度最低的实验操作（新奇探索测试），随后间隔较长时间再开展第二项测试（免疫抗性检测）。