List of degenerate primers used for DPMT.

aFor each oligonucleotide, CORE nucleotides are in bold and CLAMP sequences in normal lettering. Underlined codons do not encompass all the possible variability to avoid excessive degeneracy. The sequences used are biased to accommodate the DNA sequences of existing elements.bPrototype plasmid for the given MOB subfamily.cAmplicon size obtained from the prototype plasmid relaxase gene.dNucleotide coordinates of the prototype plasmid contained in the corresponding amplicon. An asterisk indicates that the relaxase gene is encoded in the complementary strand.a. 对于每条寡核苷酸（oligonucleotide），核心核苷酸（CORE nucleotides）以粗体标注，钳位序列（CLAMP sequences）以常规字体呈现。为避免过度简并，下划线标记的密码子未涵盖所有可能的变异类型。本次使用的序列经过偏好性调整，以适配现有元件的DNA序列。 b. 对应给定MOB亚家族的原型质粒。 c. 由该原型质粒的松弛酶基因扩增得到的扩增子大小。 d. 对应扩增子中包含的原型质粒核苷酸坐标。星号表示该松弛酶基因编码于互补链上。