RNA-seq gene expression profiling of livers from wild-type male and female mice, and from mice with a Double Knockout (DKO) of Ezh1 and Ezh2.

PolyA-selected RNA isolated from livers of adult male and female mice with a combined loss of EZH1 and EZH2 in hepatocytes was analyzed by RNA-seq. The DKO mouse model used in these analyses is a global deletion of Ezh1 and hepatocyte-specific deletion of Ezh2, and is described in Bae WK, Kang K, Yu JH, Yoo KH, Factor VM, Kaji K, Matter M, Thorgeirsson S, Hennighausen L. FASEB J. 2015 May;29(5):1653-62. doi: 10.1096/fj.14-261537. PMID: 25477280 Overall design: Liver RNA, polyA-selected, was isolated from 7 week old male and female DKO mice (Ezh1-/-, Ezh2-f/f_Alb-Cre) and their floxed (f) controls. RNA samples from n=3 mouse livers were pooled to make each of 3 biological replicate libraries per sex and genotype. AC, Albumin-Cre

本研究通过RNA测序（RNA-seq），对肝细胞中联合缺失EZH1与EZH2的成年雌雄小鼠肝脏组织所分离得到的聚腺苷酸筛选RNA（polyA-selected RNA）进行了分析。本研究采用的双基因敲除（DKO）小鼠模型为Ezh1全基因敲除联合肝细胞特异性Ezh2敲除，其构建细节参见文献：Bae WK、Kang K、Yu JH、Yoo KH、Factor VM、Kaji K、Matter M、Thorgeirsson S、Hennighausen L，FASEB J. 2015 May;29(5):1653-62，DOI：10.1096/fj.14-261537，PubMed编号（PMID）：25477280。
实验整体设计：从7周龄的双基因敲除小鼠（Ezh1-/-，Ezh2-f/f_Alb-Cre）及其floxed（f）对照小鼠的肝脏中分离聚腺苷酸筛选RNA。每3只小鼠的肝脏RNA样本混合为一个生物学重复文库，按性别与基因型分别构建3个生物学重复文库。AC即白蛋白-Cre（Albumin-Cre）。