Coupled amplification and sequencing of genomic DNA.
收藏PubMed Central1991-04-01 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC51330/
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资源简介：
Addition of dideoxyribonucleotides during the exponential phase of the PCR should result in the synthesis of two complementary sequence ladders. We have explored this hypothesis to develop coupled amplification and sequencing of genomic DNA. Coupled amplification and sequencing is a biphasic method for sequencing both strands of template as they are amplified. Stage I selects and amplifies a single target from the genomic DNA sample. Stage II accomplishes the sequencing as well as additional amplification of the target using aliquots from the stage I reaction mixed with end-labeled primer and dideoxynucleotides. We have successfully applied coupled amplification and sequencing to a 300-base-pair fragment 4 kilobases upstream from HOX2B directly from human whole genomic DNA. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-04-01
