Zenodo Data Dataset 1. TaDa-Poll gene clusters
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(Clusters tab). The Cluster column corresponds to the identity of the cluster to which the gene belongs. MeanT1, MeanT2 and MeanT3 correspond to the mean log2 ratio change for binding across the annotated gene (DamPol II/Dam) and biological replicates at T1, T2 and T3 timepoints respectively. (FDR values tab). The nomenclature Tx_y is used to represent the different biological replicates, with: x = timepoint and y = replicate. The value of the FDR (False Discovery Rate) is written for each gene for each biological replicate. Its value will determine whether the gene is considered as expressed (“1”, FDR < 0.01) or not (“0”, FDR ≥ 0.01) at each timepoint. (DAVID analysis T1/T2/T3 specific tabs). The Functional annotation clustering from DAVID for genes only found at T1, T2 or T3 yielded clusters of annotations, numbered in the Cluster column. As DAVID uses a range of annotations, the exact category and related specific term are listed within each cluster. The Cluster enrichment score corresponds to the geometric mean (in -log scale) of genes's p-values for one annotation cluster. The Count column represents the number of genes form the dataset involved in this annotation category, and the Percentage column the corresponding percentage. The p-values of each annotation term are modified Fisher Exact p-values (EASE score) and ranges from 0 to 1. p-values equal to or smaller than 0.05 are considered strongly enriched in the annotation categories. The Fold enrichment column refers to the enrichment of the annotation term compared to the background population of genes.
(Clusters标签页):Cluster列对应基因所属聚类的唯一标识。MeanT1、MeanT2及MeanT3分别对应T1、T2、T3时间点下,覆盖注释基因(DamPol II/Dam)的结合信号及其生物学重复(biological replicates)的平均log2比值变化量。 (FDR值标签页):采用Tx_y命名规则指代不同的生物学重复,其中x代表时间点,y代表重复批次。针对每个基因的每一组生物学重复,均会记录其错误发现率(False Discovery Rate,FDR)数值。该数值将用于判定基因在对应时间点的表达状态:当FDR < 0.01时记为"1"(视为表达),当FDR ≥ 0.01时记为"0"(视为不表达)。 (DAVID分析T1/T2/T3专属标签页):DAVID针对仅在T1、T2或T3时间点检出的基因开展功能注释聚类分析,生成了多组注释聚类,聚类编号将展示于Cluster列中。由于DAVID涵盖多类注释体系,每个聚类内均会列出具体的分类类别及相关特异性术语。聚类富集得分对应单个注释聚类中所有基因p值的几何均值(以-log尺度转换)。Count列代表该注释分类所涉及的数据集内基因总数,Percentage列则对应其占总数据集基因的比例。每个注释术语的p值均为校正后的费希尔精确检验p值(EASE score),取值范围为0至1。当p值≤0.05时,即可认为该注释分类存在显著富集。Fold enrichment列代表该注释术语相较于基因背景群体的富集倍数。



